Micro-Sample Techniques
(Activated Carbon, HILIC, ERLIC, RPC & SEC) microSPE columns & 96-Well platesConventional SPE Cartridge Operating Instructions
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HILICON® iSPE® HILIC SPE Cartridges
BioPureSPE™ TARGA® Fast Equilibrating, water wettable RPC.
BioPureSPE™ PROTO™ peptide protein RPC
Spin Column Operating Instructions
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ConSep™ Antibody Concentration and Desalting Columns (115µL --> 20µL without membrane concentration or spinning).
HisSep™ Histidine Removal from Antibody Formulations
BioPureSPN™ SEC Affinity Ligand Screening or Desalting SPE columns & plates
Glycan SPE
Activated Carbon: BioPureSPN Graphite for Glycan Clean-up. For additional details see Free Patent for Glycan Clean-up.
BioPureSPN™ PSA™ ERLIC MACRO HILIC
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35-350µg capacity (ERLIC) [blue] HMM PSA, primary - secondary amine HILIC price list
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17-170µg capacity (ERLIC) [blue] HEM PSA, primary - secondary amine HILIC price list
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7-70µg capacity (ERLIC) [blue] HUM PSA, primary - secondary amine HILIC price list
BioPureSPN™ MACRO HILIC
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35-350µg capacity (ERLIC) HMM HIL, PolyHYDROXYETHYL A™ HILIC price list
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17-170µg capacity (ERLIC) HEM HIL, PolyHYDROXYETHYL A™ HILIC price list
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7-70µg capacity (ERLIC) HUM HIL, PolyHYDROXYETHYL A™ HILIC price list
HILIC (...HIL, PolyLC®): Hydrophilic Interaction and Detergent Removal
HILIC cleanup of N-labelled glycans NEB Rapid PNGase F from 2AB, or procainamide reagent, NaCNBH4, DMSO & acetic acid labelling solution (with thanks to Paula Magnelli, New England Biolabs).
Unbonded Silica normal phase HILIC
Desalting
BioPureSPN™ MACRO RPC
- Color Coded, Storable Desalting Columns, 35-350µg capacity (protein C4 [yellow] HMM S04V, PROTO™ 300 C4; peptide C18 [green] HMM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HMM S18R) price list
- Color Coded, Storable Desalting Columns, 17-170µg capacity (protein C4 [yellow] HEM S04V, PROTO™ 300 C4; peptide C18 [green] HEM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HEM S18R) price list
- Color Coded, Storable Desalting Columns, 7-70µg capacity (protein C4 [yellow] HUM S04V, PROTO™ 300 C4; peptide C18 [green] HUM S18V, PROTO™ 300 C18; & [red] Fast Equilibrating TARGA® C18, HUM S18R) price list
MiniSpin™ RPC (...SS04V & ...SS18V, PROTO™ 300): Reversed phase peptide protein desalting
MiniSpin RP-sm (...SS18R, TARGA® C18): Polar molecule, hydrophobic desalting or enrichment from 100% water
BioPureSPN™ MACRO SEC and BioPureSPE™ SEC-60 instructions
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Pre-Swelled, 20% MeOH preservative, SEC Desalting Columns, 10-100µL capacity (for protein desalting) price list
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POLYHYROXYETHYL A™ SEC-60 Fractionating 96-Well Plate, 10-30µL loading price list
MiniSpin SEC (G10-G100, P2 and P6) dry resins (MACROspin recommended for capacity).
Glycopeptide Enrichment
ERLIC-WAX (...HIL-DE, PolyLC): Phosphopeptide Enrichment (ASMS poster)
BioPureSPN™ PSA™ ERLIC MACRO HILIC
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35-350µg capacity (ERLIC) [blue] HMM PSA, primary - secondary amine HILIC price list
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17-170µg capacity (ERLIC) [blue] HEM PSA, primary - secondary amine HILIC price list
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7-70µg capacity (ERLIC) [blue] HUM PSA, primary - secondary amine HILIC price list
BioPureSPN™ MACRO HIL-WAX
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35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WAX, PolyWAX LP™ WAX price list
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17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WAX, PolyWAX LP™ WAX price list
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7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WAX, PolyWAX LP™ WAX price list
Activated Carbon: BioPureSPN Graphite for phosphopeptide clean-up from salts and less polar peptides.
IMAC Phosphopeptide Enrichment (See also: S. Loroch et al., Biochimica et Biophysica Acta 1854 (2015) 460–468 Multidimensional ERLIC for ... phosphoproteome)
SCX (...HIL-SCX, PolyLC): Strong Cation Exchange: post ITRAC® & ICAT® clean-up, or subtractive phosphopeptide enrichment.
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Peptide Protein IEX or ERLIC:
ERLIC-WAX (...HIL-DE, PolyLC): Phosphopeptide Enrichment (ASMS poster)
BioPureSPN™ PSA™ ERLIC MACRO HILIC
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35-350µg capacity (ERLIC) [blue] HMM HIL-PSA, primary - secondary amine HILIC price list
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17-170µg capacity (ERLIC) [blue] HEM HIL-PSA, primary - secondary amine HILIC price list
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7-70µg capacity (ERLIC) [blue] HUM HIL-PSA, primary - secondary amine HILIC price list
BioPureSPN™ MACRO HIL-WAX
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35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WAX, PolyWAX LP™ WAX price list
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17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WAX, PolyWAX LP™ WAX price list
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7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WAX, PolyWAX LP™ WAX price list
IMAC Phosphopeptide Enrichment: no longer available (See also: S. Loroch et al., Biochimica et Biophysica Acta 1854 (2015) 460–468 Multidimensional ERLIC for ... phosphoproteome)
SCX (...HIL-SCX, PolyLC): Strong Cation Exchange: post ITRAC® & ICAT® clean-up, or subtractive phosphopeptide enrichment. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
BioPureSPN™ MACRO HIL-SCX
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35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-SCX, PolySULFOETHYL A™ SCX price list
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17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-SCX, PolySULFOETHYL A™ SCX price list
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7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-SCX, PolySULFOETHYL A™ SCX price list
WAX Weak Anion Exchange (...HIL-DE, PolyLC) organic solvent compatible for ERLIC. For ERLIC or HILIC on these columns, substitute this HILIC protocol instead.
BioPureSPN™ MACRO HIL-WAX
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35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WAX, PolyWAX LP™ WAX price list
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17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WAX, PolyWAX LP™ WAX price list
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7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WAX, PolyWAX LP™ WAX price list
BioPureSPN™ MACRO HIL-WCX
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35-350µg capacity (ERLIC) 0.2-1mg IEX; HMM HIL-WCX, PolyCAT A™ WCX price list
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17-170µg capacity (ERLIC) 100-500µg IEX; HEM HIL-WCX, PolyCAT A™ WCX price list
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7-70µg capacity (ERLIC) 40-200µg IEX; HUM HIL-WCX, PolyCAT A™ WCX price list
Protein IEX:
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SAX Strong Anion Exchange (Sepharose® Q: limited to aqueous mobile phases only)
SCX Strong Cation Exchange (Sepharose® S: limited to aqueous mobile phases only)
WAX Weak Anion Exchange (Sepharose® DEAE: limited to aqueouss mobile phases only)
WCX Weak Cation Exchange (Sepharose® CM: limited to aqueous mobile phases only)
Overview & Prices for All Chemistries and Configurations
- Spin-Con microDialysis Ultra-filtration
for 1-5µl samples
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