Amino Acid Specific Peptide Capture Kits

Biomolecular Technologies, Inc.

Cation Exchange for 2-D HPLC

2-D HPLC, PolyLC SCX Capillary Columns

eHILIC (ERLIC) for 2-D HPLC

2-D HPLC, PolyLC PolyWAX LP™ WAX Capillary Columns

Reversed Phase Capillary Columns and LC-MS Products

HAISIL RS, PROTO™ 200 C4 & C18 for Peptide Applications.
Comparison of PROTO™ 200 to Grace/Vydac® C18 for mapping or for synthetic peptides.
HAISIL RS, PROTO™ 300 C4 & C18 for Protein Applications.
HAISIL TS, TARGA ® Water-wettable, C8 & C18 for Polar Analytes & Proteomics Capture Applications.

Comparison of TARGA to BetaBasic™ for Polar Peptides
HAISIL CS, CLIPEUS™ CN, Phenyl, C8, & C18 phases, Excellent for Small Peptide Applications.

Heavily Coated (Type B Silica) Phases:
HAISIL VS, HEAVY™ Ultra-high density coating for wide pH range applications.
HAISIL HL, High Load High density coating for Fast LC Analyses.
HAISIL PS, PHALANX™ Water-wettable, Ultra-high density coating for wide pH range Polar Analytes Applications.

Strong Cation Exchange for 2-D HPLC Proteomics

Strong Cation Exchange (SCX) precolumn fractionation coupled directly to RPC-MS-MS simplifies complex protein digest characterization for increased productivity. Whether Multidimensional Chromatographic Analysis of Proteins (MudPIT), Signature Peptides, the DALPC method (Direct Analysis of Large Protein Complexes), ICAT™ (isotope-coded affinity tagged) labeled, or specific amino acid containing peptides are desired from digested proteins for proteomic characterization, this SCX column in tandem with a capillary RPC column allows true 2-D chromatographic fractionation of 1,500-2000 peptides per run at 5 orders of magnitude better detectability than 2-D gel electrophoresis.

Use of the 200Å pore SCX packings will allow the isolation of +1 peptides (C-terminal, blocked N-terminal and phosphopeptides) in the first three peaks after injection, prior to starting the salt gradient for off-line 2-D fractionation. Use of the 4.6mmID x 50mm column affords >1mg capacity with the added benefit of allowing a greater number of injections before requiring the cleaning of trypsin from the column. The 2.1mmID x 50mm SCX column would allow > 0.2mg capacity. For information of these products see our complete PolyLC price list.

PolySULFOETHYL Aspartamide SCX, (silica) is Excellent for post ICAT or DALPC* fractionation for Proteomics!

ERLIC for Selective Isolation of Phosphopeptides:
At pH 2.0, phosphate groups in peptides retain some of their negative charge. This does not permit the isolation by anion-exchange chromatography of singlely phosphorylated peptides from tryptic digests, since the electrostatic attraction is not sufficient to overcome the electrostatic repulsion from the N-terminus and the C-terminal Lys- or Arg- residue toward the basic functional groups of the column. However, phosphate residues are quite hydrophilic.

In the eHLIC mode, the combination of electrostatic attraction and hydrophilic interaction suffices to pull singly phosphorylated peptides away from the non-phosphorylated peptides in tryptic digests, at pH 2. Also, unlike the situation with high-affinity media such as IMAC or titania, the phosphopeptides are well-resolved from each other. This permits their convenient separation into numerous fractions, an important tool in phosphoproteomics for identifying the sequences of thousands of phosphopeptides from a single sample.