Instrument:         HP1090 with Diode Array
Column:              Targa C18 5µm 100x2.1mm
                             P/N: H TS-1021-C185
Mobile Phase:    3% Water in MeOH
Flowrate:             0.2mL/min
Sample:               Vitamin Mix, 2µL injection
Column Temp:     40° C
Detector:              DAD, Optimized Wavelength for each compound

Wavelength Optimization:
Vitamin K3 - 250nm, Vitamin A - 325nm, Vitamin D3 - 265nm, Vitamin E - 290nm, Vitamin K1 - 245nm

Higgins Analytical is grateful to David Isom of Analytical Sales and Services  for sharing these data.