Trap Columns
Trap Columns
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Volume Dependent Results for Retention with Partition Column Chemistries:
Desorption increases with an excess of sample loading volume and/or wash volume. The maximum loading volume is one chromatographic void volume (ca. 60% of the bed volume) less than the k' of the analyte of interest. Increased loading can be obtained by either selecting a more retentive solvent condition or from a more retentive column chemistry such as TARGA C18, which allows loading in 100% water and which could even retain (for a few column volumes) underivatized simple sugars.
TARGA C18 equilibrates up to 10x faster than other RPC columns for shorter LC-MS cycle times which saves instrument costs.
_TARGA_300ux30mm_trap.jpg)
Effect of excessive loading and wash volumes on Trap Columns
Data, courtesy of Brian Hampton, University of Maryland, generated from a trapping experiment on a 0.3 x 30mm, TARGA C18, capillary trap. Various volumes of sample in 0.1% formic acid loaded onto a trap column equilibrated with 0.1% formic acid.
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Last Updated: 08/20/21
