Nucleobond AX Properties and Applications

• Introduction
• Procedure for Cartridge Reuse
• Modified Alkaline Lysis Procedure for the Purification of Plasmids and Cosmids
• Special Conditions for Purifying P1 Constructs and Other Low Copy Number Plasmids and Cosmids
• Special Conditions for Plasmid Preparation from Gram-Positive Bacteria
• Nuclease-Free Procedure for the Purification of Bacteriophage lambda DNA and Other Single- and Double-Stranded Viral Nucleic Acids
• Recovery of DNA from Low-Melting-Temperature Agarose Gels: Re-Melt and Filter Procedure
• Recovery of DNA from Low-Melting-Temperature Agarose Gels: Freeze/Thaw in Phenol Procedure
• Isolation of Different RNAs from Cell Cultures and Tissues: Guanidine Thiocyanate Procedure
• Isolation of Total RNA (and Genomic DNA <70 Kb) from Cell Cultures and Tissues: Proteinase K Procedure Using a Plasmid Kit
• Procedure for the Purification of Oligonucleotides (<100 bp)
• Procedure for the Purification of Double-Stranded DNA (>100 bp) from Reactions
• Procedure for the Purification of PCR Products
• Procedure for the Purification of RNA from Reactions
• Purification of Genomic DNA From Prokaryotes
• Purification of Genomic DNA from Cell Cultures and Tissues: Proteinase K Procedure Using a Plasmid or a Lambda Kit
• Sequencing Methodology for Double Stranded DNA using dye-labelled primer
• Sequencing Methodology for Double Stranded DNA with fl-15-dATP
• Sequencing Solution Buffer Preparation
• N and L Series Buffer Preparation for Normal Processing of Nucleobond AX Cartridges
• N5 and L5 Elution Buffer Preparation for High GC Content DNA
• Nucleobond AX Trouble Shooting Guide

Having specific problems? Please refer to the Nucleobond AX Trouble Shooting Guide.

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