Hydrophilic Interaction Chromatography (HILIC): Gentamycin (aminoglycosides) Antibiotics LC-MS/MS
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HILIC of Gentamycin (aminoglycosides) Antibiotics With LC-MS/MS Detection.
McGrane et al., EuroResidue IV (May 2000), , Pg. 765 (van Ginkel & Ruiter, eds.), Bilthoven, The Netherlands.).
Gentamycin (aminoglycosides) Antibiotics
Figure 5.1.8: (McGrane et al., EuroResidue IV (May 2000), Pg. 765 (van
Ginkel & Ruiter, eds.), Bilthoven, The Netherlands.)
Chromatogram showing the separation achieved for aminoglycosides
HPLC Conditions ; Column : PolyHYDROXYETHYL™ Aspartamide,
200 x 4.6 mm, 5 µm, 1000Å.
Mobile phase gradient ; 80 % acetonitrile, 20 % 250
mM NH4Ac, pH 4 at t=0,
@ 1.0 ml min-1,
25 % acetonitrile, 75 % 250 mM NH4Ac, pH 4 at t=5 min.
25 % acetonitrile, 75 % 250 mM NH4Ac, pH 4 at t=15 min.
80 % acetonitrile, 20 % 250 mM NH4Ac, pH 4 at=18 min.,
Injection volume ; 50 µl
Poster: Use of 1000Å PolyHYDROXYETHYL™ Aspartamide for these very polar molecules reduced the amount of salt necessary for elution.
Chromatogram showing the separation achieved for aminoglycosides
How HILIC works
Other HILIC References
PolyHYDROXYETHYL
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Last Updated: 08/20/21
