Monoclonal Antibody Variant Analysis

Monoclonal Antibody Variant Analysis Monoclonal Antibody Variant Analysis

Advances with our PolyAspartic Acid (PolyCAT A®) columns make it possible to separate and quantitate many Monoclonal antibodies (MAb's) form variants. A common deletion is the first residue at the C-terminus of the heavy chain. That residue is always Lys- or Arg-. In the example, the third major peak is the intact MAb. The second major peak is the variant minus the terminal Lys- or Arg- from one heavy chain; the first major peak is missing the terminal residues from both heavy chains. The minor peaks represent more extensive deletions or other variations such as oxidation and the like.

Many MAb's are glycosylated, with the branches terminating in sialic acid. The number of sialic acid residues can vary. The activity of the MAb is sometimes crucially dependent on the degree of glycosylation and sialylation variants. Generally, the best conditions to use are cation-exchange on PolyAspartic Acid (1000- or 1500-Å pore diameter), with a shallow gradient of increasing pH and salt concentration. The optimal pH must be determined anew with each MAb.

Mab Variant Graphic

Improved performance can be obtained with a 1500Å pore: Column: PolyCAT A (1500Å); p/n: P1850-15-204 or 204CT0515 Gradient: 38-180 mM NaCl, pH 6.2, with 10% MeCN

Ordering Information

Polyaspartic Acid WCX column (5µ; 1000Å PolyCAT A)

Dimensions         Part Number    (PolyLC p/n)
200 x 4.6mm           P204CT0510        204CT0510
200 x 2.1mm           P202CT0510        202CT0510
100 x 4.6mm           P104CT0510        104CT0510
100 x 2.1mm           P102CT0510        102CT0510

Polyaspartic Acid WCX column (5µ; 1500Å PolyCAT A)

Dimensions         Part Number     (PolyLC p/n)
200 x 4.6mm           P204CT0515        204CT0515
200 x 2.1mm           P202CT0515        202CT0515
100 x 4.6mm           P104CT0515        104CT0515
100 x 2.1mm           P102CT0515        102CT0515

For other part numbers and prices, please see the PolyLC Price List .

For more information contact:
The Nest Group, Inc.™ 17 Hayward St., Ipswich, MA 01938-2041 USA
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Last Updated: 08/20/21