A phospholipid assay was developed to allow direct injection of a QC sample of mixed phospholipids and analysis by ELSD or MS detection using volatile solvents. No sample preparation was necessay. Comparison to a HILIC method

showed that the elution was controllable and that there was no interference from the solvent peak at the end of the eHILIC gradient:

While the 301A-VHP575 chemistry has been obsoleted, an alternative eHILIC column, PolyWAX LP™, P054WX0510, will approximate this method. However, use 1.0 mL/min and a 25µL injection as a modification of the conditions shown above.