Below is a chronological list of events:
09/01/24- After 40 years since starting The Nest Group, Inc. Amos retired. If you need technical support email him at
webmaster to save him from retirement boredom. This site will remain active as a resource. Please contine to use our Nest Group part numbers when publishing since only shipping is reverting to our manufacturers.
11/17/23 - Updated link
Buffer Preparation Calculator (by Professor Robert J Beynon).
09/29/23 - Added
Detergents & Buffers for HIC or IEX separations.
11/03/22 - Added
Affinity Ligand Screening or Desalting SPE columns & plates
05/16/22 - Added
"EasyFlo" RPC, 96-Well Vacuum Desalting Plates and 96-Well 0.45µm Outlet Positive Pressure/Centrifuge 96-Well Plates.
08/20/21 - Added 1mmID iHILIC®(P)-Classic column availability
1mmID HILIC prices.
06/10/21 - Relative Strength of Salts on
Retention in HILIC publication.
06/03/21 - Added 5-850µL Empty BioPureSPN Columns with 10µm frits,
C-100500, to replace Page-Erasers (SUM 0007), Empty µ-Permethylation Reactors /w caps (SUM 0000-BW) & Empty Macro-Permethylation Reactors (SMM 0000).
10/22/20 - Linked
Nature Protocols, (2020)
Glycomic Chacterization. Recommends iSPE® HILIC for (Steps 100–117) Glycoproteomic analysis: protein digestion, enrichment, and MS analysis.
10/20/20 - Added
Cardiolipin Isolation Publication (
https://doi.org/10.1002/rcm.8566) which outlines a HILIC-based clean‐up method enabling complete separation of polar lipids containing four fatty acyl residues from non-polar lipid classes using iSPE® HILIC columns.
01/21/20 - Added ID Biologics™ High Capacity-High Recovery mAbSep™
Antibody Desalting Kits
10/30/19 - Added
iHILIC®-(P) Classic 5µm, 200Å columns for metabolomics, inorganic ions and nTP methods developed on ZIC® pHILIC columns.
5/30/19 - Added
RNA-ALIS: Methodology for screening soluble RNAs as small molecule targets.
5/29/19 - Added
SEC-Native MS Protein-Conjugate Affinity Screening.
5/09/19 - Added Drug-Protein Conjugate Combinatorial Library Screening using
SEC-RPC narrowbore columns on LC-MS systems.
3/12/19 - Added Monitoring
glutamine, glutamate,
& glufosinate metabolomics
2/22/19 - Added
iHILIC® Publications
01/25/19 - Added
BioPureSPN HIL-PSA HILIC-Amine part numbers and operating instructions for glycopeptide enrichment or 2-D ERLIC fractionation of peptide digests.
12/13/18 - Added operating instructions for the
iHILIC®-(P) Classic
11/5/18 - Added multiple bed mass
BioPureSPE Scout-96-Well Plates
10/14/18 - LCGC article on Practical Methods for
Improving LC–MS Sensitivity
10/10/18 - Added BioPureSPN HILIC-PSA SPE
Primary Secondary Amine ERLIC or IEX
08/28/18 - Added
iSPE glycopeptide enrichment data from "Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation" paper.
08/13/18 - Added BioPureSPN HILIC-IEX SPE
Parts & Protocols
08/10/18 - Added ID Biologics™ Expanded Capacity HisSep-250™
Histidine Removal Protocol for Antibody Formulations
07/18/18 - Added high-affinity, iron-chelating
Siderophore (Biometal compounds) separations with iHILIC® Fusion
07/02/18 - Added iHILIC® Fusion
Phospholipid Class Separations
06/12/18 - Added BioPureSPN Graphite SPE
Phosphopeptide & Glycan isolation
11/13/17 - Added iHILIC® Fusion(+) separation of
mono- & di-saccharides glucose, fructose, sucrose, maltose
10/23/17 - Added
HIC LC-MS for Top Down
Proteomics
08/08/17 - Added volatile buffer
HIC-LC-MS columns
07/30/17 - Added
SEC-LC-MS with volatile buffers publication
06/22/17 - Added
Direct Analysis of Amino Acids by HILIC–ESI-MS
05/02/17 - Added Comparison of Zwitterionic HILICON® HILIC columns
Fusion to
Fusion(+) for LC-MS of underivatized amino acids
03/17/17 - Added Non-Ferrous Columns
Piccolo™, Sprite™, HAIPEEK™ cartidges
02/14/17 - Added Effect of Buffer Strength When Separating
cGAMP or Di- and Tri-phosphates
12/14/16 - Added
semi-reusable BioPureSPN™
micro-desalting & particle removal columns for LC-MS samples.
10/14/16 - Added iHILIC®
Fusion(P) polymeric columns for inorganic ion analyses.
06/28/16 - Added
ERLIC outperforms TiO
2-MOAC in quantitative phosphoproteomics with low sample amounts. Comparison of
PolyWAX LP® to
titanium dioxide
06/26/16 - Added
ConSep™ Antibody Concentration and Desalting Columns (115µL --> 20µL without membrane concentration).
06/01/16 - Added iHILIC®
Fusion(P) polymeric columns to substitute for pHILIC columns for high pH metabolomic separations.
04/20/16 - Added HILIC separation of
UDP-Glucose on iHILIC® Fusion column.
04/18/16 - Added HILIC separation of
Artificial Sweetners: Sucralose, Acesulfame K, Saccharin, Neotame, Neohesperidin, Na Cyclamate, & Aspartame.
03/30/16 - Added
HisSep a spin column product optimized for the rapid and gentle removal of histidine from antibody formulation buffers.
03/22/2016 - Added
Permethylation µ-Reactors /w caps for glycan solid-phase labelling
method.
3/18/2016 - Added
Cleanup of N-labelled glycans by HILIC SPE.
12/12/2015 - Added
iHILIC & iSPE Fusion & Fusion(+) columns & and Cartridge Products for metabolomics. Up to 2000 metabolite IDs in 20 minutes.
10/21/2015 - Added 2015
Higgins Catalog (7.8 MB pdf) of HAI
SIL™ HPLC Columns, µTrap™, Ca
πllary™ & Capellini™ capillary columns & and Cartridge Products.
04/05/15 - Added
Buffer Preparation Calculator (University of Liverpool). Choose the buffer species you want to use. Enter parameters for volume, pH, and concentration of buffer species, with an option to modify the ionic strength by addition of neutral salt. Finally, enter the temperature at which you'll use the buffer, and the temperature at which you'll make it up. All in all and excellent tool you'll find useful.
03/20/15 - Added
Column Portfolio for ADC Characterization overview.
11/15/14 - Added
The Importance of Detector Response Time when using Superficially Porous Packings: If not fast enouogh, at low concentrations, narrow peaks appear to give non-linear responses.
9/26/14 - Added Eliminate tailing due to metals chelating sample on frits or MS emitter tips with a 50mM disodium EDTA treatment (an off-line, non-volatile treatment), or more conveniently, with acetylacetonate in the mobile phase.
8/19/14 - Added
Removal of phospholipids using HILIC on silica SPE cartridges with acetone as eluent for eliminating matrix effects in the analysis of biological fluids when using RPC LC-MS.
6/24/14 - Added
Volatile Buffers for LCMS & ELSD systems.
4/30/14 - Added
Antibody Analyses using Zenix SEC & WCX NP5 columns.
3/25/14 - Added
Antibody Drug Conjugate (ADC) RP-1000 Analyses
10/25/13 - Added
Antibody Drug Conjugate (ADC) Analysis
04/12/13 - Added recent
ERLIC-WAX Publications in a Dropbox.
01/20/13 - Added
Selectivity changes in HILIC by manipulating pH and buffer strength.
11/13/12 - Added ERLIC-WAX SPE
Phosphopeptide Enrichment Instructions and
ASMS Poster containing ERLIC-WAX Tryptic Phosphopeptide Fractionation supporting data.
11/08/12 - Added
ERLIC-MS Peptide Deamidation detection
11/07/12 - Added
ERLIC for shotgun proteomics
11/06/12 - Added Pi
3MET-TRYP
Application for selective methionine capture for Thermodynamic analysis of protein- Ligand interactions.
10/25/2012 - Added ZIC®-
cHILIC brochure for complementary selectivity for Polar Separations.
10/25/2012 - Added ZIC-HILIC
Brochure for Polar Separations.
10/3/2012 - Added an example of a
Polyvalent, Unimolecular Glycopeptide Separation Using PROTO300 C4.
6/29/2012 - Added
Size Exclusion: A theoretical basis
5/15/2012 - Added Organic acids to probe the effects of
pH and coulombic interaction of HILIC phases.
10/28/2011 - Added Complete
Higgins Catalog (3.67 MB pdf) of HAI
SIL™ HPLC Columns, µTrap™, Ca
πllary™ & Capellini™ capillary columns & and Cartridge Products.
8/18/2011 - Volume Dependent Results for Retention with Partition Columns:
Effect of excessive loading volume on SPE Tips or Trap Columns. Use of TARGA C18 to increase retention of polar molecules for quantitative proteomics experiments
4/2/2011 - An evaluation of an inverted zwitterionic,
ZIC-cHILIC, cholinate stationary phase for proteomic 2-D identification of more than 20,000 unique peptides corresponding to over 3,500 proteins.
3/25/2011 - Added link to "How to
Polish Capillary Ends" to reduce nano-flow chromatographic system dead volume in tubing connections.
2/11/2011 - Added
Molarity of Concentrated Acids & Bases: Dilutions to Make 1 Molar Solutions.
10/21/2010 - Added
Sepax SEC-C Columns Provide a Wider SEC Range of Separation on Their SRT-C and Zenix-C (lay-down) SEC Phases.
6/28/2010 - Added
Orientation Effects of Electrostatic Repulsion HILIC (also known as e-HILIC and Ion-Pair Normal Phase) to ERLIC section.
3/15/2010 - Added
Wolfram|Alpha™ a computational knowledge engine which contains information on chemical elements, unit conversions, flash points, thermodynamic properties, reactivity, and much more to
Chemistry Links. Based on a new kind of knowledge-based computing Wolfram|Alpha is the first step in an ambitious, long-term project to make all systematic knowledge immediately computable by anyone. Enter your
chemical question or calculation and Wolfram|Alpha uses its built-in algorithms and a growing collection of data to compute the answer.
3/11/2010 - Added EMD Chemicals' monolithic columns,
Chromolith™
1/4/2010 - Added An Introduction to Ion Chromatography and trouble shooting manual:
"A Practical Guide to Ion Chromatography"
8/17/2009 - Added Knitted Open Tubular (KOT) Reaction Delay & Mixing Coil
information.
8/8/2009 - Added Complete
Higgins Catalog (3.67 MB pdf) of HAI
SIL™ HPLC Columns and Cartridge Products.
7/01/2009 - Added
µ-Reactors™ with caps to remove solid reactants from micro quantities of samples. Xia B., et al. Anal Biochem. 2009 Apr 15;387(2):162-70. Epub 2009 Feb 10.
Glycan reductive isotope labeling (GRIL) for quantitative glycomics.
6/18/2009 - Added a new, eHILIC method for
glycopeptide separations from high mannose type and sialylated glycopeptides.
6/16/2009 - Added a link to a masters thesis showing a capillary RPC method for separating
small RNAs from post translational modifications.
4/15/2009 - Added a new, low protein loss, fast technique for excess
Dye Removal from protein.
4/1/2009 - Added a HILIC
Newsletter-0309. Discusses alternatives to ACN for HILIC assays and announces the U.S. HILIC Day prior to the ASMS meeting in Philadelphia.
2/15/2009 - Added a HILIC
Newsletter-0109. Discusses diethylene glycol analysis in medicine, and suggestions on how to reduce consumption of acetonitrile.
1/26/2009 - Added a new HILIC
Newsletter-1208. The December 2008 edition discusses methyl malonic acid analysis in serum, plasma and urine plus an ELSD detection method for choline and inositol.
11/20/2008 - Added a link for a subscription to a HILIC
Newsletter-1108. The November 2008 edition discusses melamine analysis in food products and an invitation to HILIC Users Group meetings.
9/18/2008 - Added Analysis of
Melamine in food products with ZIC-HILIC columns.
8/22/2008 - Added
Retention Time Predictor for ZIC-HILIC columns.
7/29/08 - Added links for
demos of the Cerex System-48 & System-96.
7/10/08 - Added FAST MicroEquilibrium Dialyzers to the
micro-dialysis price list.
3/12/08 - Added the reference
Science,
319, 1197-98 (2008) to the
Protein HIC section for a discussion of Specific Ion Effects regarding the hydration of ions on surfaces.
2/20/08 - Added
PROTO300 C4: Comparison to Vydac 214TP C4 for proteins.
12/31/07 - Added
YMC America as a New source for a broad range of HPLC and Process scale bonded medias.
12/20/07 - Added
Comparison of the
All-Guard™ Cartridges to the Vydac "...GD54" Guard Cartridges.
11/7/07 - Added Brian Hampton's ASMS Poster Regarding The
Importance of Contact Time for Either Desalting or Trapping of Polar Solutes on Trap Columns.
11/1/07 - Added
KOT Knitted Open Tubular Reactors to price list.
7/14/07 - Added
ERLIC Discussion of Electrostatic Repulsion Effects in HILIC separations.
2/14/07 - Added
Newly Designed Index, Protocols and Personnel pages. We hope you like it better
12/5/06 - Added
PROTO™ 200 & PROTO™ 300 & Peptide & Protein PROTEO columns to
Higgins Analytical section of Price List
11/1/06 - Added
Buffer Solubility in Aqueous-Organic Solvents Useful for RPC and/or HILIC mobile phase composition selection.
8/25/06 - Added
Dialysis Bibliography to
Dialysis section of Protocols
8/10/06 - Added [sic] "HPLC Method for the Determination of Pharmaceutical Salts" to
ZIC-HILIC section of Protocols
5/15/06 - Added
Basics of HPLC to
Training section of Protocols
5/12/06 - Added
WWW Chemistry Guide - a collection of links for organic, biochemical, computational, medicinal and other chemistry research or education.
5/2/06 - Added 110nL capillary
LC/MS Guard/Traps to Higgins Analytical Piccolo™ price list
7/20/05 - Added a search engine to the site for searching within The Nest Group URL, www.nestgrp.com .
7/6/05 - Added
ZIC-pHILIC® for Adenosine Phosphate Analysis (AMP, cAMP, dAMP, ADP, dATP and ATP)
Using HILIC Conditions with Volatile Buffers (70% ACN, 30% Ammonium Carbonate, pH 8.8, 100 mM).
5/6/05 - Added
comparison (Y. Guo & S. Gaiki, J. Chromatogr. A, 1074 (2005) 71-80.) of
Amide, Amine, Silica and betain sulfonate functional groups for HILIC separations of small polar acids, nucleic acid bases and nucleosides.
4/9/05 - Added Oleg Krokhin's Peptide
RT Calculator. New sequence-specific
correction factors for prediction of peptide retention in RP-HPLC: application to protein identification by off-line HPLC-MALDI-MS.
3/19/05 - Added references for
measurement of pH in aqueous/organic solutions
3/7/05 - Updated
PolyLC Bibliographic references
2/04/05 - Added this ExPASy link in Chemistry WWW Resources to
Calculate pKa
and provide other information resources for life scientists with an interest
in biological macromolecules. Links to protein sequence, 3D structure and 2D-gel analytical tools are provided on the
ExPASy server, and more specifically from its
Proteomics tools page.
11/01/04 - Added this link in Chemistry WWW Resources. The
Hofmeister Effect and
the implications for HIC. This site discusses the ranking of various ions toward their ability to precipitate a mixture of proteins.
06/03/04 - Added pdf HILIC Comparison to RPC for
Metabolomics.
03/05/04 - Added slides:
Designing a purification protocol using ion exchange resins.
10/17/03 - Added chromatograms on
Aminoglycoside (Gentamycin) Antibiotic separations in the Protocols Index under
Polar Molecule and Detergent Removal by HILIC.
4/18/03 - Added a series of articles on
Mobile-Phase Buffer considerations in the Protocols Index under
Training.
4/1/03 -
New Everest™ C18
Ultra High Resolution Tryptic Peptide Mapping Columns. LC-MS/MS Capillaries, Guards and Analytical Columns.
3/15/03 - Added
New Higgins Capellini™, Piccolo™ and Sprite™
LC-MS/MS Capillaries, Guards and Trap Columns.
9/19/2002 -
HILIC LC-MS/MS of Folic Acid Metabolites and Underivatized Amino Acids.
7/8/2002 -
PAGE*Eraser™ microFilter Spin Columns remove particulates from samples from in-gel digests to prolong capillary LC-MS column life.
6/20/2002 - Added Catalog of HAI
SIL® HPLC Columns and Cartridge Products. Complete (2,668K pdf) or individual sections:
HAISIL 100®
(small pore) and
HAISIL 300®
(wide pore) products (Type A Silicas); and
TS
TARGA ®, CS
CLIPEUS® and
LS "High Load" products (Type B Silicas).
4/8/2002 - Added
Hydrophobic Interaction (HIC) part numbers and prices.
4/5/2002 -
Dialysis membrane conditoning and solvent compatibility information.
3/6/2002 - Proteomics applications newsletter on low TFA MS Vydac media using prepacked
capillary columns from 0.075mmID - 0.500mmID.
2/14/2002 -
Training lecture: Basics of Dialysis and Ion Exchange, added to Nest Protocols Section
2/13/2002 -
Orbigen Biology Protocols link added to WWW Biology Sites section
1/22/2002 -
Biology Online link added to our WWW Biology Sites information section.
10/31/2001 - New!
Grace Vydac 0.075 and 0.150mmID Capillary Columns
for "Low TFA" LC-MS operation enhace detection of peptides.
9/10/2001 -
Proteomics: Pi
3™ TYR-P Poster data. Isolation
of tyrosine-phosphate containing peptides from digests
5/1/2001 -
Proteomics Fractionation: Pi
3™ amino acid
specific resins for complex sample fractionation
3/14/2001 - HIC and IEX column care added to IdeaBook and Protocols sections for PolyLC products.
3/1/2001 -
2-D chromatographic fractionation of 1,500-2000
peptides per run at 5 orders of magnitude better detectability than 2-D gel electrophoresis whether you are using
Multidimensional Chromatographic Analysis of Proteins (
MUDCAP), Signature Peptides, the
DALCPC method
(Direct Analysis of Large Protein Complexes), ICAT™ (isotope-coded affinity tagged)
labeled, or isolating specific amino acid containing peptides from digested proteins
for proteomic characterization.
1/10/2001 - The Nest Group URL established to utilize cgi scripting and dynamic HTML.
8/22/2000 - Pdf technical sheets were integrated with the
ideabook
section for all products.
1/12/2000 - Updated all products with new prices.
3/18/99 -
MiniSpin
Columns and MicroTip Kits were added.
9/1/98 -
Biology
Reference sites were added.
6/10/98 -
DispoBioDialyser
AmiKa's DispoBioDialyser ® disposable, small volume (10-100 ul) dialysis
kits added.
6/10/98 -
Cozap
AmiKa's Cozap® Coomassie Blue destaining products were added.
11/15/96 - New,
EXCLU-SIEVE®
Agaroses are added to the protocols section.
2/1/96 - The Nest Group, Inc. now accepts credit card orders for
your convenience.
1/31/96 - The Nest Group, Inc. URL received the Magellan 4 Star
Award.
12/13/95 - Vydac RPC Buffer Note "Validation of Reverse Phase Chromatography
separations (TFA, PO4, HOAc, HCl)" was added.
11/7/95 - Nucleobond AX®, NucleoTrap®, NucleoTraPCR®,
NucleoSpin®, NucleoSpin Extract®, and NucleoSpin Blood Extract®
part numbers were changed from
MN 730... to MN 740...
8/15/95 - Nucleobond AX® DNA sequencing protocols added.
8/3/95 - Protein & Peptide HPLC Columns technical information
is now on-line.
7/25/95 - The NucleoTrap®, NucleoTraPCR®, NucleoSpin®,
and NucleoSpin Extract® properties and applications guides were added.
7/25/95 - The Nucleobond AX® properties and applications guide
was added.
6/14/95 - The Vendor page was added.
5/27/95 - The molecular biology WWW resource page was added.
5/26/95 - Literature request and feedback forms were implemented.
4/26/95 - The Nest Group, Inc. goes on-line with the opening of
this Web site.
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